Part:BBa_K1172915:Design

Biosafety-System TetOR alive (TetR)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1156
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1080
  Illegal BamHI site found at 1782
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1198
  Illegal AgeI site found at 1498
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 955
  Illegal BsaI.rc site found at 2774
  

Design Notes

• Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Prefix_Insertion Prefix-Insertion] of the Biobrick BBa_K1172912 into the Backbone of the BioBrick BBa_K1172914.

Source

• Part 1 of the Biosafety-System TetOR alive BBa_K1172912
  
• Part 2 of the Biosafety-System TetOR alive BBa_K1172914 containing GFP (BBa_E0040) instead of the RNase Ba (BBa_K1172904)